Metabolomic analysis will be performed at Penn State University under the direction of Andrew D. Patterson, PhD.
The Penn State Metabolomics Facility has two hybrid Quadrupole Time-of-Flight (Q-TOF) tandem mass spectrometers, an AB SCIEX 5600 Triple TOF with a Shimadzu Prominence UFLC, a Thermo Scientific EXACTIVE PLUS with a Dionex UHPLC, and a Waters Synapt G2-S with a Waters Acquity UPLC. We have recently acquired a Thermo Scientific Orbitrap Fusion Lumos Tribrid with a Vanquish UHPLC and a Bruker AVANCE NEO 600 MHz NMR with SampleJet. These instruments are used for global profiling and identification of the metabolites present in biofluids and tissue extracts.
Two triple quadrupole mass spectrometers (Waters Xevo TQ-S, and Waters Xevo TQ-D, both with Waters Acquity UPLCs) are used for LC-MS/MS based targeted analysis and for validation and accurate quantitation of compounds identified in the global profiling approach.